{"product_id":"biolegend-366507","title":"Biolegend, 366507, APC anti-human CD257 (BAFF, BLYS) Antibody, 25tests","description":"\u003cp\u003eBAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA\/ionomycin treatment.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD257 transfected murine cell line.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hase H, et al. 2004. Blood 6:2257. (IHC)\u003cbr\u003e\nProduct Citations: Chappert P, et al. 2022. Immunity. 55:1872. PubMed\u003cbr\u003e\nRRID: AB_2565710 (BioLegend Cat. No. 366507) AB_2565711 (BioLegend Cat. No. 366508)\u003cbr\u003e\nStructure: Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.\u003cbr\u003e\nDistribution: Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA\/ionomycin treatment.\u003cbr\u003e\nFunction: Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR\/BR3).\u003cbr\u003e\nLigand\/Receptor: BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR\/BR3).\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokines\/Chemokines, Growth Factors, Soluble Receptors\u003cbr\u003e\nAntigen References: 1. Schneider P, et al. 1999. J. Exp. Med. 189:1747. 2. Moore PA, et al. 1999. Science 285:260. 3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680. 4. Yu G, et al. 2000. Nat. Immunol. 1:252. 5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.\u003cbr\u003e\nGene ID: 10673\u003cbr\u003e\nUniProt: View information about CD257 on UniProt.org\u003cbr\u003e\nClone: 1D6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860855640233,"sku":"366507","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-366507","provider":"Iright","version":"1.0","type":"link"}