{"product_id":"biolegend-372102","title":"Biolegend, 372102, Purified anti-human CD172a (SIRPα) Antibody, 100μg","description":"\u003cp\u003eCD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes\/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KG1a cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Majdic O, et al. 1981. Blood. 58:1127.\u003cbr\u003e\nProduct Citations: Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed\u003cbr\u003e\nRRID: AB_2629807 (BioLegend Cat. No. 372102)\u003cbr\u003e\nStructure: Type I transmembrane protein, 85-90 kD, one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, two ITIM motifs and a proline-rich region in the cytoplasmic tail.\u003cbr\u003e\nDistribution: Monocytes, macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells.\u003cbr\u003e\nFunction: Negative regulation of receptor tyrosine kinase-coupled signaling, mediates macrophage multinucleation, involved in neuronal survival.\u003cbr\u003e\nLigand\/Receptor: CD47, SP-A, and SP-D.\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Lv Z, et al. 2015. J. Immunol. 195:661. 2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024. 3. Zen K, et al. 2013. Nat. Commun. 4:2436. 4. Baba N, et al. 2013. J. Exp. Med. 210:1251. 5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457.\u003cbr\u003e\nGene ID: 140885\u003cbr\u003e\nUniProt: View information about CD172alpha on UniProt.org\u003cbr\u003e\nClone: 15-414\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BIT, MFR, P84, MYD-1, SHPS1, PTPNS1, CD172 antigen-like family member A\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860810715305,"sku":"372102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-372102","provider":"Iright","version":"1.0","type":"link"}