{"product_id":"biolegend-372222","title":"Biolegend, 372222, Alexa Fluor® 700 anti-human\/mouse Granzyme B Recombinant Antibody, 100tests","description":"\u003cp\u003eGranzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm \/ 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Yang C, et al. 2023. Front Immunol. 14:1112672. PubMed Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed Dean JW 2023. Cell Reports. 42(1):111963. PubMed Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed Moon S, et al. 2021. J Exp Med. 218:. PubMed Trivedi S, et al. 2020. Elife. 9:00. PubMed\u003cbr\u003e\nRRID: AB_2728388 (BioLegend Cat. No. 372221) AB_2728389 (BioLegend Cat. No. 372222)\u003cbr\u003e\nStructure: 32 kD serine protease\u003cbr\u003e\nDistribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells\u003cbr\u003e\nFunction: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA\/ IL-2 and CD4+ T lymphocytes with anti CD3\/CD28 or CD3\/CD46.\u003cbr\u003e\nInteraction: Caspase-3\u003cbr\u003e\nLigand\/Receptor: Mannose-6-phosphate receptor\u003cbr\u003e\nCell Type: T cells, NK cells, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Proteases, Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.\u003cbr\u003e\nGene ID: 14939\u003cbr\u003e\nUniProt: View information about Granzyme B on UniProt.org\u003cbr\u003e\nClone: QA16A02\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866168840361,"sku":"372222","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-372222","provider":"Iright","version":"1.0","type":"link"}