{"product_id":"biolegend-372751","title":"Biolegend, 372751, PerCP\/Fire™ 780 anti-human TIGIT (VSTM3) Antibody, 25tests","description":"\u003cp\u003eT cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Human TIGIT.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Fire™ 780 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Fire 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).Additional reported applications (for the relevant formats) include: Blocking1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Stamm H, et al. 2018. Oncogene. Pubmed\u003cbr\u003e\nRRID: AB_3083361 (BioLegend Cat. No. 372751) AB_3083361 (BioLegend Cat. No. 372752)\u003cbr\u003e\nStructure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.\u003cbr\u003e\nDistribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.\u003cbr\u003e\nFunction: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.\u003cbr\u003e\nLigand\/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).\u003cbr\u003e\nCell Type: NK cells, T cells, Tfh, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858. 2. Yu X, et al. 2009. Nat. Immunol. 10:48. 3. Johnston R, et al. 2014. Cancer Cell. 26:923.\u003cbr\u003e\nGene ID: 201633\u003cbr\u003e\nUniProt: View information about TIGIT on UniProt.org\u003cbr\u003e\nClone: A15153G\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864388456617,"sku":"372751","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-372751","provider":"Iright","version":"1.0","type":"link"}