{"product_id":"biolegend-372804","title":"Biolegend, 372804, PE anti-human CD133 Antibody, 100tests","description":"\u003cp\u003eCD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant partial human CD133 protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This clone can block the staining of AC133 clone in flow cytometry.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Swaminathan SK, et al. 2010. J. Immunol. Methods 361:110.\u003cbr\u003e\nProduct Citations: Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed Deshmukh A, et al. 2018. Cancers (Basel). 10. PubMed Wan J, et al. 2021. Front Pharmacol. 11:590723. PubMed Wang HC, et al. 2022. J Biomed Sci. 29:99. PubMed Chang S, et al. 2020. Cell Death Dis. 0.869444444. PubMed Sahin ID, et al. 2020. Sci Rep. 10:11814. PubMed Duan X, et al. 2022. Cell Biosci. 12:60. PubMed Hinz S, et al. 2021. iScience. 24:103026. PubMed Wu S, et al. 2020. Exp Ther Med. 1.988888889. PubMed Saga R, et al. 2019. J Radiat Res. 60:298. PubMed Ho CM, et al. 2022. Am J Cancer Res. 12:1686. PubMed Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed King P, et al. 2021. Int J Oncol. 59:. PubMed Huang R, et al. 2020. Cell Death Dis. 11:234. PubMed Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed Hirao A, et al. 2021. Cancers (Basel). 13:. PubMed\u003cbr\u003e\nRRID: AB_2632879 (BioLegend Cat. No. 372803) AB_2632880 (BioLegend Cat. No. 372804)\u003cbr\u003e\nStructure: 120 kD pentaspan transmembrane glycoprotein.\u003cbr\u003e\nDistribution: Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells\u003cbr\u003e\nCell Type: Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Yin AH, et al. 1997. Blood. 90:5002. Miraglia S, et al. 1997. Blood. 90:5013. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.\u003cbr\u003e\nGene ID: 8842\u003cbr\u003e\nUniProt: View information about CD133 on UniProt.org\u003cbr\u003e\nClone: clone 7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prominin-1, AC133 Antigen, PROM1, PROML1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862992801961,"sku":"372804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-372804","provider":"Iright","version":"1.0","type":"link"}