{"product_id":"biolegend-373750","title":"Biolegend, 373750, StarBright UltraViolet 740 anti-human TIGIT (VSTM3) Antibody, 100tests","description":"\u003cp\u003eThis StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative .  This product is also available for direct purchase within a StarBright Basic Panel , which combines a minimal set of pre-selected compatible reagents. T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Human TIGIT.\u003cbr\u003e\nFormulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm. StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.\u003cbr\u003e\nExcitation Laser: Ultraviolet Laser (355 nm)\u003cbr\u003e\nApplication Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).Additional reported applications (for the relevant formats) include: Blocking1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Stamm H, et al. 2018. Oncogene. Pubmed\u003cbr\u003e\nStructure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.\u003cbr\u003e\nDistribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.\u003cbr\u003e\nFunction: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.\u003cbr\u003e\nLigand\/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).\u003cbr\u003e\nCell Type: NK cells, T cells, Tfh, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858. 2. Yu X, et al. 2009. Nat. Immunol. 10:48. 3. Johnston R, et al. 2014. Cancer Cell. 26:923.\u003cbr\u003e\nGene ID: 201633\u003cbr\u003e\nUniProt: View information about TIGIT on UniProt.org\u003cbr\u003e\nClone: A15153G\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What is a “StarBright panel”?\u003cbr\u003e\nA: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.\u003cbr\u003e\nQ: Will my StarBright panel be provided as a cocktail?\u003cbr\u003e\nA: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Are StarBright GMP\/ASR products available?\u003cbr\u003e\nA: StarBright ASR and GMP products are coming soon.\u003cbr\u003e\nQ: Are StarBright custom products available?\u003cbr\u003e\nA: Yes, please contact our Custom Solutions Team.\u003cbr\u003e\nQ: Will StarBright isotype controls be offered?\u003cbr\u003e\nA: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.\u003cbr\u003e\nQ: What amount of a StarBright conjugate should I use?\u003cbr\u003e\nA: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.\u003cbr\u003e\nQ: Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?\u003cbr\u003e\nA: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864390586537,"sku":"373750","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-373750","provider":"Iright","version":"1.0","type":"link"}