{"product_id":"biolegend-376213","title":"Biolegend, 376213, Brilliant Violet 421™ anti-human TNF-α Antibody, 25tests","description":"\u003cp\u003eTNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix\/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nRRID: AB_3097480 (BioLegend Cat. No. 376213) AB_3097480 (BioLegend Cat. No. 376214)\u003cbr\u003e\nStructure: TNF superfamily; dimer\/trimer; 17 kD (Mammalian)\u003cbr\u003e\nDistribution: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells\u003cbr\u003e\nFunction: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists\u003cbr\u003e\nInteraction: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia\u003cbr\u003e\nLigand\/Receptor: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)\u003cbr\u003e\nCell Type: B cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, NK cells, NKT cells, T cells, Th1, Th17\u003cbr\u003e\nBiology Area: Adaptive Immunity, Cell Death, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Volova L.T, et al. 2020. Biomolecules. 10:1563. Szondy Z, et al. 2017. Pharmacological Research. 115:124-132.\u003cbr\u003e\nGene ID: 7124\u003cbr\u003e\nUniProt: View information about TNF-alpha on UniProt.org\u003cbr\u003e\nClone: W19063E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866343395497,"sku":"376213","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-376213","provider":"Iright","version":"1.0","type":"link"}