{"product_id":"biolegend-377123","title":"Biolegend, 377123, PE anti-mouse\/human TOX Antibody, 100μg","description":"\u003cp\u003eTOX, a member of the high-mobility group (HMG) box superfamily of DNA-binding proteins, was originally identified as a thymic transcript expressed in CD4 + CD8 + double-positive T cells. Along with TOX2, TOX3, and TOX4, TOX is also part of a small subfamily of transcription factors that share conserved HMG-box sequences. TOX is highly upregulated in thymocytes undergoing positive selection and is required for the differentiation and development of various T cell subsets, including CD4 + T cells, regulatory T cells, and natural killer T cells (NKTs). TOX-deficient mice fail to develop lymph nodes and have significantly diminished numbers of CD4 + T, NK, and ILC cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse TOX recombinant protein (21-236 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Use of True-Nuclear™ Transcription Factor or True-Phos™ Perm Buffer are recommended for TOX detection with clone W17072A Clone W17072A does not block the staining of reference clones TXRX10, REA473 and NAN448B tested.\u003cbr\u003e\nStructure: 562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: TOX is a transcription factor required for the development of various T lymphocyte subsets.\u003cbr\u003e\nInteraction: TOX interacts with ZDHHC17\u003cbr\u003e\nBiology Area: Transcription Factors\u003cbr\u003e\nAntigen References: Spits H. 2015. Nat Immunol. 16:594. Seehus CR, et al. 2015. Nat Immunol. 16:599. Huang Y, et al. 2015. Blood. 125:1435. Aliahmad P, et al. 2012. Curr Opin Immunol. 24:173. Di Santo JP. 2010. Nat Immunol. 11:885. Aliahmad P, et al. 2004. J Exp Med. 199:1089.\u003cbr\u003e\nGene ID: 252838\u003cbr\u003e\nUniProt: View information about TOX on UniProt.org\u003cbr\u003e\nClone: W17072A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Thymocyte Selection-Associated High Mobility Group Box, Thymus High Mobility Group\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866312593577,"sku":"377123","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-377123","provider":"Iright","version":"1.0","type":"link"}