{"product_id":"biolegend-378003","title":"Biolegend, 378003, Ultra-LEAF™ Purified anti-human CD20 (Rituximab Biosimilar, RUO) Recombinant Antibody, 100μg","description":"\u003cp\u003eCD20 is a surface marker expressed on B lymphocytes. CD20 is a 33-37 kD protein with four hydrophobic transmembrane domains that it associates with cell-surface and cytoplasmic proteins such as CD53, CD81, CD82, MHCII, CD40, CBP, and BCR. This association contributes to signal transduction essential for the development, differentiation, and activation of B cells. CD20 is also variably expressed by B cell malignancies such as Chronic lymphocytic leukemia (CLL), follicular lymphoma, and diffuse large B-cell lymphoma (DLBCL).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Human\u003cbr\u003e\nImmunogen: Human CD20\u003cbr\u003e\nFormulation: 150 mM sodium chloride, pH 6.5\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested CMCD - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For Complement-Dependent Cytotoxicity assays the IC50 range is 0.2 - 0.5 µg\/mL. It is recommended that the reagent is titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This recombinant antibody is based on the published sequence for the Rituximab antibody. This product is for research use only. It is not intended for therapeutic or diagnostic purposes.\u003cbr\u003e\nRRID: AB_2924569 (BioLegend Cat. No. 378003) AB_2924569 (BioLegend Cat. No. 378004)\u003cbr\u003e\nStructure: CD20 protein has four membrane-spanning domains with both the amino and carboxy termini facing the cytosol\u003cbr\u003e\nDistribution: B cell, T cell subset\u003cbr\u003e\nFunction: The function of CD20 involves the development, differentiation, and activation of B cells\u003cbr\u003e\nInteraction: CD20 associates with CD53, CD81, CD82, MHCII, CD40, CBP, BCR, and Src family kinases\u003cbr\u003e\nLigand\/Receptor: The ligand for CD20 is unknown\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Li H, et al. 2003. J Biol Chem. 278:42427-34. Pavlasova G, et al. 2020. Haematol. 105:1494-1506. Kuek E, et al. 2016. Immunol Cell Biol. 94:11-23.\u003cbr\u003e\nGene ID: 931\u003cbr\u003e\nUniProt: View information about CD20 on UniProt.org\u003cbr\u003e\nClone: QA20A02\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, MS4A1, Leukocyte surface antigen Leu-16, membrane-spanning 4-domains superfamily A member 1\u003cbr\u003e\nIsotype: Human IgG1, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866190532777,"sku":"378003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-378003","provider":"Iright","version":"1.0","type":"link"}