{"product_id":"biolegend-381502","title":"Biolegend, 381502, Purified anti-Puromycin Antibody, 100μg","description":"\u003cp\u003ePuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Puromycin from Streptomyces alboniger coupled to KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested ICC, WB, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 2.5 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 1.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg\/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase. For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.\u003cbr\u003e\nRRID: AB_2924575 (BioLegend Cat. No. 381502)\u003cbr\u003e\nDistribution: Cytoplasm, Ribosomes\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Protein Synthesis\u003cbr\u003e\nAntigen References: Schmidt E, et al. 2009. Nat Methods. 6:275-7. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Puromycin on UniProt.org\u003cbr\u003e\nClone: 2A4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: puromycin from streptomyces alboniger\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866552520873,"sku":"381502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-381502","provider":"Iright","version":"1.0","type":"link"}