{"product_id":"biolegend-388708","title":"Biolegend, 388708, APC anti-human CD8a Antibody, 100tests","description":"\u003cp\u003eCD8α, also known as T8 or Leu2, is a 32-34 kD type 1 transmembrane glycoprotein part of the immunoglobulin superfamily. CD8α is expressed on cytotoxic T cells, NK cells, as well as a majority of thymocytes. CD8α can form a homodimer (CD8α\/α) or heterodimer (CD8α\/β) with CD8β. CD8α acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8α are important for function: the extracellular Ig superfamily domain binds the α3 domain of MHC Class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD8α-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone S21010A can completely block the binding of clone HIT8a on target cells and can be blocked by HIT8a. Clone S21010A does not block the binding of clone SK1, clone RPA-T8, and clone S21010F. Clone S21010A does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40. Clone S21010A shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.\u003cbr\u003e\nRRID: AB_3717109 (BioLegend Cat. No. 388707) AB_3717109 (BioLegend Cat. No. 388708)\u003cbr\u003e\nStructure: Ig superfamily\u003cbr\u003e\nDistribution: Subset of T cells, most thymocytes, NK cells\u003cbr\u003e\nFunction: TCR co-receptor\u003cbr\u003e\nInteraction: CD8β\u003cbr\u003e\nLigand\/Receptor: MHC Class I molecules\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego. Leahy, D.J, 1995. FASEB J. 9:17-25.\u003cbr\u003e\nGene ID: 925\u003cbr\u003e\nUniProt: View information about CD8alpha on UniProt.org\u003cbr\u003e\nClone: S21010A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Leu2, CD8 Antigen\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866272092329,"sku":"388708","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-388708","provider":"Iright","version":"1.0","type":"link"}