{"product_id":"biolegend-390905","title":"Biolegend, 390905, PE anti-human CD16 Antibody, 25tests","description":"\u003cp\u003eCD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human CD16 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone W18182A does not block the binding of clones 3G8, B73.1, and W18182B on target cells.\u003cbr\u003e\nRRID: AB_3662289 (BioLegend Cat. No. 390905) AB_3662289 (BioLegend Cat. No. 390906)\u003cbr\u003e\nStructure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)\u003cbr\u003e\nDistribution: Expressed on natural killer cells, activated monocytes, macrophages, neutrophils and a subset of T cells\u003cbr\u003e\nFunction: Low affinity IgG Fc receptor\u003cbr\u003e\nLigand\/Receptor: IgG Fc (Fcγ)\u003cbr\u003e\nCell Type: Granulocytes, Neutrophils, NK cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Schubert J, et al. 1989. Oxford University Press. pp 711. Ravetch JV and Bolland S. 2001. Annu Rev Immunol. 19:275-90.\u003cbr\u003e\nGene ID: 22142215\u003cbr\u003e\nUniProt: View information about CD16 on UniProt.org\u003cbr\u003e\nClone: W18182A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866335039657,"sku":"390905","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-390905","provider":"Iright","version":"1.0","type":"link"}