{"product_id":"biolegend-395902","title":"Biolegend, 395902, Purified anti-human IRF3 Antibody, 100μg","description":"\u003cp\u003eIRF-3 is a roughly 50 kD member of the interferon regulatory transcription factor (IRF) family. Along with other members of the IRF family IRF-3 plays a key role in the innate immune response to viral infection. It is located ubiquitously in an inactivated form in the cytoplasm of many immune cells. When phosphorylated it forms a complex with CREBBP to upregulate many immune responses integral to innate immune function.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IRF-3 protein (entire protein)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Rustagi A, et al. 2012. Methods. 59(2):225-32\u003cbr\u003e\nRRID: AB_2810597 (BioLegend Cat. No. 395902)\u003cbr\u003e\nStructure: Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.\u003cbr\u003e\nDistribution: Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine\/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.\u003cbr\u003e\nFunction: CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776 Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654 Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12\u003cbr\u003e\nGene ID: 3661\u003cbr\u003e\nUniProt: View information about IRF3 on UniProt.org\u003cbr\u003e\nClone: AR1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IRF3, IRF-3, interferon regulatory factor 3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862541553833,"sku":"395902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-395902","provider":"Iright","version":"1.0","type":"link"}