{"product_id":"biolegend-398402","title":"Biolegend, 398402, Purified anti-human CD32B\/C Antibody, 100μg","description":"\u003cp\u003eCD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of human CD32B\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Su K, et al. 2007. J Immunol. 178:3272\u003cbr\u003e\nRRID: AB_2814383 (BioLegend Cat. No. 398402)\u003cbr\u003e\nStructure: Type I transmembrane protein, 40 kDa, member of the Ig superfamily\u003cbr\u003e\nDistribution: B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.\u003cbr\u003e\nFunction: Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation\u003cbr\u003e\nLigand\/Receptor: Fc region of complex or aggregated immunoglobulins gamma\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Monocytes, Platelets\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Bruhns P, et al. 2009. Blood. 113: 3716. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735. Descours B, et al. 2017. Nature 543: 564. Tomiyama Y, et al. 1992. Blood. 80: 2261. Indik Z, et al. 1991. J Clin Invest. 88: 1766. Ramsland PA, et al. 2011. J Immunol. 187: 3208. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311. Bournazos S, et al. 2009. J Immunol. 182: 8026. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437. Sandilands GP, et al. 1997. Immunology. 91: 204. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878. Gillis C, et al. 2014. Front Immunol. 5: 254.\u003cbr\u003e\nGene ID: 22139103\u003cbr\u003e\nUniProt: View information about CD32B CD32C on UniProt.org\u003cbr\u003e\nClone: 4F5\/CD32\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862416019625,"sku":"398402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-398402","provider":"Iright","version":"1.0","type":"link"}