{"product_id":"biolegend-400531","title":"Biolegend, 400531, PerCP\/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl Antibody, 25μg","description":"\u003cp\u003eThe RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)\u003cbr\u003e\nProduct Citations: Murikinati S, et al. 2009. FASEB J. 24:788. PubMed Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed Li J, et al. 2022. Nat Commun. 13:4032. PubMed Takizawa S, et al. 2022. Mol Med. 28:91. PubMed Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed Tal R, et al. 2019. Biol Reprod. 100:1228. PubMed Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed Zhang M, et al. 2019. Autophagy. 1.423611111. PubMed\u003cbr\u003e\nRRID: AB_2864286 (BioLegend Cat. No. 400531) AB_2864286 (BioLegend Cat. No. 400532)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: RTK2758\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866206818473,"sku":"400531","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-400531","provider":"Iright","version":"1.0","type":"link"}