{"product_id":"biolegend-400723","title":"Biolegend, 400723, PerCP\/Cyanine5.5 Rat IgG2c, κ Isotype Ctrl Antibody, 25μg","description":"\u003cp\u003eThe isotype of RTK4174 immunoglobulin is rat IgG2c, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this rat IgG2c, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed\u003cbr\u003e\nRRID: AB_2864281 (BioLegend Cat. No. 400723)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: RTK4174\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Rat IgG2c, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866210357417,"sku":"400723","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-400723","provider":"Iright","version":"1.0","type":"link"}