{"product_id":"biolegend-405235","title":"Biolegend, 405235, Alexa Fluor® 488 Streptavidin, 100μg","description":"\u003cp\u003eStreptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 488 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 488 by 488 nm laser light induces a light emission maximum of 519 nm.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Streptavidin was conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml (concentration relates to the Streptavidin only component of the conjugate)\u003cbr\u003e\nStorage \u0026amp; Handling: The streptavidin-Alexa Fluor® 488 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 488 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Streptavidin-Alexa Fluor® 488 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 488 is 60 kD and Streptavidin alone is 52 kD.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chappaz S, et al. 2007. Blood doi:10.1182\/blood-2007-02-074245. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed Yamakawa N, et al. 2012. Int Immunol. PubMed Shibata T, et al. 2012. Int Immunol. 24:613. PubMed Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed Reading JL, 2013. J. immunol. 190:4542. Pubmed. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed\u003cbr\u003e\nProduct Citations: Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed Tran JR, et al. 2022. Nucleic Acids Res. 50:e117. PubMed Melzi E, et al. 2022. Immunity. 55:2168. PubMed Peng W, et al. 2022. Cell Mol Life Sci. 79:602. PubMed Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Churchill MJ, et al. 2022. J Exp Med. 219:. PubMed Han Y, et al. 2020. Vaccines (Basel). 8:00. PubMed Tran JR, et al. 2021. J Cell Biol. 220: . PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Evans R, et al. 2019. Cell Rep. 27:1967. PubMed Aneas I, et al. 2021. Nat Commun. 12:6115. PubMed Tomi\u0026amp;cacute; S, et al. 2016. Sci Rep. 6:31618. PubMed Tomi\u0026amp;cacute; S, et al. 2017. Biomaterials. 10.1016\/j.biomaterials.2017.08.040. PubMed Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed Alameh MG, et al. 2021. Immunity. 54:2877. PubMed Delacher M, et al. 2020. Immunity. 52:295. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Lederer K, et al. 2022. Cell. . PubMed Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed Tichy ED, et al. 2021. Cell Rep. 35:109098. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-Alexa Fluor® 488, Streptavidin-AF488, SAV\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866503499945,"sku":"405235","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-405235","provider":"Iright","version":"1.0","type":"link"}