{"product_id":"biolegend-405716","title":"Biolegend, 405716, APC\/Cyanine7 anti-mouse IgD Antibody, 100μg","description":"\u003cp\u003eSurface IgD is an important B cell differentiation marker.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed Ke F, et al. 2023. Elife. 12:. PubMed Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed Turner J, Benet Z, and Grigorova I. 2017. J Immunol. 10.4049\/jimmunol.1700267. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Proudhon C, et al. 2016. Cell Rep. 15:2159-2169. PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Reynolds A, et al. 2015. J Immunol. 194:231. PubMed Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed Wilmore JR et al. 2018. Cell host \u0026amp; microbe. 23(3):302-311 . PubMed Prados A, et al. 2021. Nat Immunol. 22:510. PubMed Saumyaa S, et al. 2013. Infect Immun . 81:3426. PubMed Webster P, et al. 2018. Nat Commun. 9:2649. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Alameh MG, et al. 2021. Immunity. 54:2877. PubMed Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed Xiao G, et al. 2018. Cell. 173:470. PubMed Scally S, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01924-3. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Palmer V, et al. 2015. J Immunol. 195: 1524-1537. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed Niemeyer BF, et al. 2021. PLoS Pathog. 17:e1010019. PubMed Laczkó D, et al. 2020. Immunity. 53:724. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_10660304 (BioLegend Cat. No. 405715) AB_10662544 (BioLegend Cat. No. 405716)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 380797\u003cbr\u003e\nUniProt: View information about IgD on UniProt.org\u003cbr\u003e\nClone: 11-26c.2a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin D\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860784500905,"sku":"405716","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-405716","provider":"Iright","version":"1.0","type":"link"}