{"product_id":"biolegend-406410","title":"Biolegend, 406410, Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity) Antibody, 125microl","description":"\u003cp\u003eThis polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Rabbit\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Donkey\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICC - Validated\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunocytochemistry, the suggested usage of this reagent is a 1:20-1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.\u003cbr\u003e\nProduct Citations: Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Jin J, et al. 2023. Nat Aging. 3:600. PubMed Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed Muto M, et al. 2016. J Am Soc Nephrol. 10.1681\/ASN.2016050496. PubMed Doehl JSP, et al. 2022. Front Immunol. 12:795554. PubMed Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed Madge P, et al. 2016. Sci Rep. 6:36012. PubMed Canton J, et al. 2021. Nat Immunol. 22:140. PubMed Cao W, et al. 2016. Nat Commun. 7:11687. PubMed Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed Tsao LC, et al. 2022. JCI Insight. . PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Vogel R, Erez A, Altan-Bonnet G 2016. Nat Commun. 7: 12428. PubMed Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed Bennett FC, et al. 2018. Neuron. 98:1170. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Lamsoul I, et al. 2012. PLoS One. 7:e43798. PubMed Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed Yamasaki T, et al. 2018. J Virol. 92:e01458. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed Winkler C, et al. 2021. JCI Insight. 6:. PubMed Bohlen CJ et al. 2017. Neuron. 94(4):759-773 . PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed Becker L, et al. 2013. PLoS One. 8:e59452. PubMed Liddelow S, et al. 2017. Nature. 541:481-487. PubMed Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed\u003cbr\u003e\nRRID: AB_10897810 (BioLegend Cat. No. 406410)\u003cbr\u003e\nGene ID: 100343867\u003cbr\u003e\nUniProt: View information about Rabbit IgG on UniProt.org\u003cbr\u003e\nClone: Poly4064\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Donkey Polyclonal Ig\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866469781673,"sku":"406410","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-406410","provider":"Iright","version":"1.0","type":"link"}