{"product_id":"biolegend-406504","title":"Biolegend, 406504, Biotin anti-mouse IgM Antibody, 500μg","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed\u003cbr\u003e\nProduct Citations: Lai M, et al. 2016. Nat Commun. 7:12207. PubMed Gonzalez-Martin A, et al. 2016. Nat Immunol. 17:433-440. PubMed Zhao M, et al. 2019. Cell Rep. 26:652. PubMed Suzuki M, et al. 2021. Sci Adv. 7:. PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed Boulianne B, et al. 2013. J Immunol. 191:4521. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed Yamada H, et al. 2018. J Immunol. 201:3492. PubMed\u003cbr\u003e\nRRID: AB_315053 (BioLegend Cat. No. 406503) AB_315054 (BioLegend Cat. No. 406504)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 16019\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: RMM-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866200920233,"sku":"406504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-406504","provider":"Iright","version":"1.0","type":"link"}