{"product_id":"biolegend-406514","title":"Biolegend, 406514, PE\/Cyanine7 anti-mouse IgM Antibody, 100μg","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed\u003cbr\u003e\nProduct Citations: Hong H, et al. 2022. STAR Protoc. 3:101526. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Erickson JJ, et al. 2022. Nature. 606:769. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed Cunha P, et al. 2022. Front Vet Sci. 9:891893. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371\/journal.pone.0185509. PubMed Sauma D, et al. 2017. Immunol Res. 10.1007\/s12026-017-8936-9. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed Staffas A et al. 2018. Cell host \u0026amp; microbe. 23(4):447-457 . PubMed Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed\u003cbr\u003e\nRRID: AB_10640069 (BioLegend Cat. No. 406513) AB_10642031 (BioLegend Cat. No. 406514)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 16019\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: RMM-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866201608361,"sku":"406514","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-406514","provider":"Iright","version":"1.0","type":"link"}