{"product_id":"biolegend-409002","title":"Biolegend, 409002, Purified anti-Biotin Antibody, 500μg","description":"\u003cp\u003eBiotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.\u003cbr\u003e\n500μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Biotinylated limulus hemocyanin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Uccellini MB, et al. 2008. J. Immunol. 181:5875. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.\u003cbr\u003e\nProduct Citations: Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Jiang S, et al. 2022. Immunity. 55:1118. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed Green N, et al. 2012. J Biol Chem. 287:39789. PubMed\u003cbr\u003e\nRRID: AB_10642032 (BioLegend Cat. No. 409002)\u003cbr\u003e\nLigand\/Receptor: Avidin, Streptavidin\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nClone: 1D4-C5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vitamin B7, Vitamin H\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860852789417,"sku":"409002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-409002","provider":"Iright","version":"1.0","type":"link"}