{"product_id":"biolegend-421203","title":"Biolegend, 421203, ELISA Assay Diluent (5X), 100ml","description":"\u003cp\u003eELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.\u003cbr\u003e\n100ml\u003cbr\u003e\nStorage \u0026amp; Handling: This solution can be stored unopened between 2°C and 8°C. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage. Warm ELISA reagents to room temperature (18-25°C) before use.\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nRecommended Usage: Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4. PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 µm filter, pH to 7.4\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed\u003cbr\u003e\nProduct Citations: Maizels K 2014. Nucleic Acids Res. 42:108. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ELISA assay diluent, ELISA diluent\u003cbr\u003e\nQ: I ran out of assay diluent provided with ELISA MAX™ Deluxe set.\u003cbr\u003e\nA: You can order more assay diluent using Cat. No. 421203.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864111075497,"sku":"421203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-421203","provider":"Iright","version":"1.0","type":"link"}