{"product_id":"biolegend-422608","title":"Biolegend, 422608, Human CD3\/CD28\/CD137 T Cell Activation Beads, VO22","description":"\u003cp\u003eThe Human CD3\/CD28\/CD137 T Cell Activation Beads allow for convenient in vitro activation of human T cells without the need for antigen-presenting cells or precoating culture plates. Human T cells are stimulated with microbeads coated with monoclonal anti-CD3, anti-CD28 and anti-CD137 antibodies. The antibodies present on the bead surface act as an agonist and stimulate T cells. This mimics antigen presenting cells to induce the proliferation, especially for memory T cells which rely on CD137 signaling for cell maintenance and activation. Activation in vitro with this product results in robust T cell expansion, especially of the central memory (CCR7 + CD45RA - ) CD8 + T cell subset.  Simply add the beads directly to isolated T cells at a 1:1 particle-to-cell ratio for robust T cell activation. The antibody-coated beads can be removed from the cells using a magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.\u003cbr\u003e\nVO22\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nFormulation: Cocktail: N\/A Particle: Phosphate buffer solution containing 0.1% HSA (azide-free). The particles are at a concentration of 4 x 107 particles\/mL. The Human CD3\/CD28\/CD137 T Cell Activation Beads are tested for microbial\/mycoplasma contamination.\u003cbr\u003e\nEndotoxin Level: Less than 10 EU\/mL as determined by the LAL test.\u003cbr\u003e\nPreparation: The antibodies were purified by affinity chromatography and conjugated with magnetic microbeads under optimal conditions.\u003cbr\u003e\nStorage \u0026amp; Handling: All components should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: Activ - Quality tested\u003cbr\u003e\nRecommended Usage: Use Human CD3\/CD28\/CD137 T Cell Activation beads at a 1:1 particle-to-cell ratio.\u003cbr\u003e\nApplication Notes: This product is designed for the activation of isolated human T cells for research use only (RUO). The antibody-coated beads can be removed from the cells by the use of a handheld magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173) or other commercially available magnetic separators. Please contact BioLegend Technical Service for more product-specific information and guidance.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T Cell Stimulation Beads, Human T Cell Stimulation Beads, Human CD3 CD28 CD137 Beads, T cell expansion beads, Memory T cell Expansion Beads\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864273113257,"sku":"422608","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-422608","provider":"Iright","version":"1.0","type":"link"}