{"product_id":"biolegend-426201","title":"Biolegend, 426201, Lymphopure™, 250ml","description":"\u003cp\u003eLymphopure™ is a ready-to-use filtered and endotoxin tested solution for the isolation of mononuclear cells (MNCs) from a cell suspension. The solution is formulated at a density that allows separation of MNCs from other cells in blood such as granulocytes and erythrocytes. Granulocytes and erythrocytes will sediment after centrifugation.\u003cbr\u003e\n250ml\u003cbr\u003e\nFormulation: Density gradient medium for the isolation of mononuclear cells formulated at a density of 1.077 ± 0.001 g\/mL, and osmolality of 290 ± 15 mOsm.\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store at room temperature protected from light.\u003cbr\u003e\nApplication: Cell Separation - Quality tested\u003cbr\u003e\nRecommended Usage: Watch a video protocol. Collect blood into a tube containing anti-coagulant (EDTA, heparin) Dilute the blood with an equal volume of phosphate-buffered saline or similar suitable medium. Mix Lymphopure™ by inverting the bottle several times. Aliquot half the amount of Lymphopure™ into a tube. For every ml of diluted blood, add 0.5 ml of Lymphopure™. Carefully layer the diluted blood on top of Lymphopure™. Avoid mixing blood with Lymphopure™. Centrifuge at 800 x g for 20 minutes at room temperature (15 - 25°C) without braking. If the blood has been stored for more than 2 hours, centrifuge for 30 minutes. After centrifugation, mononuclear cells (MNCs) form a defined cell layer at the plasma: Lymphopure™ interface. Collect the MNC layer at the interface without disturbing the other fractions. Wash MNCs with medium.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:9-29. Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:77-89. Böyum A. 1964. Nature. 204:793-4. Thorsby E, Bratile A. 1970. Histocompatibility Testing 1970,ed. P.I. Terasaki. 655-6. Ting A, Morris PJ. 1971. Vox. Sang. 20(6)561-3.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Density gradient medium\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866475155625,"sku":"426201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-426201","provider":"Iright","version":"1.0","type":"link"}