{"product_id":"biolegend-427505","title":"Biolegend, 427505, 2-Deoxy-D-glucose, VO27","description":"\u003cp\u003e2-Deoxy-D-glucose (2-DG) blocks glycolysis by inhibiting glucose-6-phosphate production. 2-DG is transported into the cell by glucose transporters where it is phosphorylated by hexokinase. Phosphorylated 2-DG cannot be further metabolized by phosphoglucose isomerase and accumulates in the cell, leading to the depletion of cellular ATP. 2-DG is used in metabolic studies to determine cellular dependency on glycolysis.\u003cbr\u003e\nVO27\u003cbr\u003e\nFormulation: Lyophilized\u003cbr\u003e\nPreparation: Resuspend 1 g of lyophilized 2-Deoxy-D-Glucose in 60.9 mL of water or PBS to make a 100 mM stock solution. For 250 mg size, add 15.2 mL water or PBS to make a 100 mM stock solution.\u003cbr\u003e\nStorage \u0026amp; Handling: Store lyophilized powder at -20°C. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid freeze thaw cycles.\u003cbr\u003e\nApplication: Cell Culture - Verified\u003cbr\u003e\nRecommended Usage: 2-Deoxy-D-glucose can be used for inhibition of glycolysis. For primary immune cells (e.g. PBMCs or splenocytes) a range of 2.5 – 10.0 mM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.\u003cbr\u003e\nStructure: C6H12O5. 164.16 g\/mol\u003cbr\u003e\nFunction: Glycolysis inhibitor\u003cbr\u003e\nAntigen References: Arguello, et al. 2020. Cell Metabolism. 32:1063-1075. Traba, et al. 2016. J Vis Exp. 117:54918. Li, et al. 2019. J Cell Phys. 234:11. Lin, et al .2017. Oncotarget. 8:110426. Repas, et al. 2022. Cancers. 14:1343.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Glycolysis Inhibitor, 2-Deoxyglucose, 2-Deoxy-D-arabinohexose, C6H12O5\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866474205353,"sku":"427505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-427505","provider":"Iright","version":"1.0","type":"link"}