{"product_id":"biolegend-430126","title":"Biolegend, 430126, FluoroSpot MAX™ Deluxe Set Human IFN-γ, 5plates","description":"\u003cp\u003eHuman IFN-γ (Interferon-gamma) is a multifunctional cytokine which is the sole member of the class II Interferon. Although a variety of immune cells can express IFN-γ, most of IFN-γ is produced by T cells and NK cells. It has critical roles in many processes including, but not limited to, anti-viral response, regulating Th1\/Th2 balance, enhancing antigen presentation, and controlling cellular proliferation and apoptosis. IFN-γ can bind to 2 receptors: IFNGR1 and IFNGR2, which transduce the signal downstream through the JAK-STAT pathway. Because of its multifunctional nature, dysregulation of IFN-γ expression and activity has been implicated in many diseases such as autoimmune and cancer.  BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of human IFN-γ secreting cells. In this assay, anti-human IFN-γ capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted IFN-γ to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represents the “footprint” of an individual IFN-γ-secreting cell,  allowing for precise quantification of cytokine-producing cells in the sample.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: FluoroSpot\u003cbr\u003e\nCell Type: B cells, Eosinophils, Macrophages, Mast cells, Neutrophils, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 3458\u003cbr\u003e\nUniProt: View information about IFN-gamma on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IFNG, IFG, IFI, interferon gamma, Immune interferon\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864323248297,"sku":"430126","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-430126","provider":"Iright","version":"1.0","type":"link"}