{"product_id":"biolegend-430224","title":"Biolegend, 430224, ELISpot MAX™ Deluxe Set Human TNF-α, 5plates","description":"\u003cp\u003eHuman TNF-α (Tumor Necrosis Factor α), also known as Cachectin, Cytotoxic Factor (CF), is a cytokine which has important roles in regulating a wide range of biological functions from immune activation, cellular differentiation, proliferation, apoptosis, and energy metabolism. It can be expressed and secreted by macrophages, neutrophils, T-cells, NK cells, and a few other cell types. TNF-α can bind to two receptors: TNFR1 and TNFR2. Signaling through TNFR1 is mostly pro-inflammatory, while TNFR2 signaling is mainly anti-inflammatory and proliferative. Because of its diverse functions, dysregulation or TNF-α expression and activity has been implicated in many diseases from autoimmune, cancer, to neurodegeneration. On the other hand, there is also interest in using TNF-α as an immunostimulant drug to enhance the anti-cancer activity of monoclonal antibodies.  BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of human TNF-α secreting cells. A monoclonal antibody specific for human TNF-α is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and\/or unstimulated cells are pipetted directly into the wells, and then incubated at 37oC for a specific period of time. During this period, capture antibody binds to human TNF-α from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-human TNF-α detection antibody is added, followed by avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of human TNF-α secreting cells. The end result is visible spots, which corresponds to footprints of TNF-α secreting cells, on the membrane surface.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: ELISpot\u003cbr\u003e\nCell Type: Macrophages, Neutrophils, NK cells, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 7124\u003cbr\u003e\nUniProt: View information about TNF-alpha on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor Necrosis Factor alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864320200873,"sku":"430224","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-430224","provider":"Iright","version":"1.0","type":"link"}