{"product_id":"biolegend-430230","title":"Biolegend, 430230, FluoroSpot MAX™ Deluxe Set Human TNF-α\/IL-6, 5plates","description":"\u003cp\u003eHuman TNF-α (Tumor Necrosis Factor α), also known as Cachectin, Cytotoxic Factor (CF), is a cytokine which has important roles in regulating a wide range of biological functions from immune activation, cellular differentiation, proliferation, apoptosis, and energy metabolism. TNF-α is expressed and secreted by macrophages, neutrophils, T-cells, NK cells, and various other cell types. TNF-α can bind to two receptors: TNFR1 and TNFR2. Signaling through TNFR1 is mostly pro-inflammatory, while TNFR2 signaling is mainly anti-inflammatory and proliferative. Because of its diverse functions, dysregulation or TNF-α expression and activity has been implicated in many diseases from autoimmune, cancer, to neurodegeneration.  Human IL-6 (Interleukin-6), also known as B-cell Stimulatory Factor 2 (BSF2), Interferon beta-2 (IFN-β-2), Hybridoma Growth Factor (HGF), is a cytokine, which is expressed by many different cell types such as macrophages, T-cells, osteoblasts, endothelial cells, and hepatocytes. It is involved in both pro-inflammatory and anti-inflammatory responses. In particular, IL-6 has critical roles in multiple different biological processes including but not limited to B-cell maturation, osteoclast formation, nerve cell differentiation, muscle healing and hypertrophy. Thus, abnormal level of expression and activity of IL-6 can result in a wide range of diseases from chronic inflammation, cancer, to neurodegenerative diseases.  BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents and buffers for the simultaneous detection of human TNF-α and IL-6 secreting cells. In this assay, anti-human TNF-α and IL-6 capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted TNF-α and\/or IL-6 to bind to nearby capture antibodies. After incubation, cells are removed, and fluorescent conjugated detection antibodies are added. The resulting spots represent the \"footprints\" of individual TNF-α and IL-6-secreting cells, enabling precise quantification of both single cytokine-producing cells and dual cytokine-producing cells within the sample.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: FluoroSpot\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Eosinophils, Macrophages, Mast cells, Neutrophils, Osteoblasts, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 71243569\u003cbr\u003e\nUniProt: View information about TNF-alpha, IL-6 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor Necrosis Factor α (TNF-α), Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864320299177,"sku":"430230","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-430230","provider":"Iright","version":"1.0","type":"link"}