{"product_id":"biolegend-430624","title":"Biolegend, 430624, ELISpot MAX™ Deluxe Set Human IL-10, 5plates","description":"\u003cp\u003eInterleukin 10 (IL-10), also known as CSIF, GVHDS, is a cytokine which plays a critical role in protecting the host from excessive responses to pathogens, while also participating in wound healing, cancer, and homeostasis. Through its binding to the IL-10 receptor, a complex of IL-10R1 and IL-10R2, IL-10 functions as an anti-inflammatory cytokine by down-regulating TH-1 cytokine and promoting development of regulatory T cells (Treg). Major sources of IL-10 include T helper cells, monocytes, macrophages, and dendritic cells. However, other cells such as B cells and epithelial cells are also capable of producing IL-10. Abnormally low level of IL-10 can cause autoimmunity and chronic inflammation (e.g. colitis), while high level of IL-10 can result in immune deficiency and tumor tolerance.  BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of human IL-10 secreting cells. A monoclonal antibody specific for human IL-10 is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and\/or unstimulated cells are pipetted directly into the wells, and then incubated at 37 ° C for a specific period of time. During this period, the capture antibody binds to human IL-10 from the secreting cells in the vicinity. Then, cells are removed and a biotinylated anti-human IL-10 detection antibody is added, followed by Avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of human IL-10 secreting cells. The end result is visible spots, which corresponds to footprints of IL-10 secreting cells, on the membrane surface.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: ELISpot\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 3586\u003cbr\u003e\nUniProt: View information about IL-10 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin 10, Cytokine synthesis inhibitory factor, CSIF, GVHDS\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864267477161,"sku":"430624","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-430624","provider":"Iright","version":"1.0","type":"link"}