{"product_id":"biolegend-439226","title":"Biolegend, 439226, FluoroSpot MAX™ Deluxe Set Human Granzyme B, 5plates","description":"\u003cp\u003eGranzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Perforin\/granzyme pathway is a molecular weapon for the defense against virus-infected and malignantly transformed host cells. Perforin protein creates a pore in the membrane of targets cells and facilitate the entry of granzymes. This results in the translocation of Granzyme B into the nucleus and initiation of apoptosis. Other granzymes have also been implicated in apoptosis, but Granzyme B appears to be the most potent. Human Granzyme B is a potential immunoconjugate and an important facilitator in chronic inflammation and wound healing.  BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of human Granzyme B secreting cells. In this assay, anti-human Granzyme B capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted Granzyme B to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represent the “footprint” of an individual Granzyme B-secreting cell, allowing for precise quantification of cytokine-producing cells in the sample.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: FluoroSpot\u003cbr\u003e\nCell Type: Basophils, Dendritic cells, Leukocytes, Lymphocytes, Macrophages, Mast cells, Monocytes, NK cells, NKT cells, T cells, Th1, Tregs\u003cbr\u003e\nBiology Area: Adaptive Immunity, Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology, Innate Immunity, Mitochondrial Function, Neuroinflammation, Signal Transduction, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, Cytokines\/Chemokines, Enzymes and Regulators, Immune Checkpoint Receptors, Innate Immune Signaling, MHC Antigens, Mitochondrial Markers, Proteases, TCRs, Toll Like Receptors, Tumor Suppressors\u003cbr\u003e\nGene ID: 3002\u003cbr\u003e\nUniProt: View information about Granzyme B on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme-2, GranzymeB, GRB, GrzB, GZMB\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866444583081,"sku":"439226","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-439226","provider":"Iright","version":"1.0","type":"link"}