{"product_id":"biolegend-480040","title":"Biolegend, 480040, MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit, 200tests","description":"\u003cp\u003eNon CD4 + Naïve T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4 + Naïve T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.  MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.  Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.\u003cbr\u003e\n200tests\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nFormulation: Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.\u003cbr\u003e\nPreparation: The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Strepavidin Nanobeads: Streptavidin-coated magnetic beads.\u003cbr\u003e\nStorage \u0026amp; Handling: Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: Cell Separation (MojoSort™) - Quality tested\u003cbr\u003e\nRecommended Usage: 10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer. 10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.\u003cbr\u003e\nApplication Notes: This kit is designed for the isolation of untouched naïve CD4+ T cells from lymphoid tissues. For optimal results, we recommend incubating the sample in PBS without Ca\/Mg on ice for 30 minutes at the final step of the sample preparation procedure. See the protocols for further instructions. Each lot has been individually optimized. Do not mix and match components from different lots or different kits. Antibody or cocktail dilution to use in column: 8X Nanobead dilution to use in columns: 5X\u003cbr\u003e\nProduct Citations: Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed Dou D, et al. 2021. Clin Sci (Lond). 135:597. PubMed Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed Guo F, et al. 2022. Appl Microbiol Biotechnol. 106:7643. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Kiritsy MC, et al. 2021. Elife. 10:. PubMed Dong L, et al. 2017. Sci Rep. 10.1038\/srep36598. PubMed Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed Duan H, et al. 2021. J Clin Invest. 131:. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed Feng P, et al. 2021. Theranostics. 11:9503. PubMed Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed Lee WH, et al. 2019. Exp Mol Med. 51:143. PubMed Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about CD4 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nQ: Is there a way to detach your magnetic particles from the cell surface?\u003cbr\u003e\nA: MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eMojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.\u003cbr\u003e\nQ: What is the size of your magnetic particles?\u003cbr\u003e\nA: MojoSort Nanobeads: the average diameter is approximately 130 nm.\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eMojoSort Microbeads: the average diameter is approximately 1 μm.\u003cbr\u003e\nQ: Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?\u003cbr\u003e\nA: MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.\u003cbr\u003e\nQ: What antibodies are present in the depletion cocktails provided for isolation kits?\u003cbr\u003e\nA: Please contact our technical service team for further assistance.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864273604777,"sku":"480040","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-480040","provider":"Iright","version":"1.0","type":"link"}