{"product_id":"biolegend-500106","title":"Biolegend, 500106, PE anti-human IL-1α Antibody, 100tests","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IL-1α\u003cbr\u003e\nFormulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture1: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.Flow Cytometry4: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Additional reported applications (for the relevant formats) include: immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Thorpe R, et al. 1988. Lymphokine Res. 2:119. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177. Lu Y, et al. 2015. PNAS. 112:607. PubMed\u003cbr\u003e\nProduct Citations: Aouba A, et al. 2010. Blood. 116:4070. PubMed\u003cbr\u003e\nRRID: AB_315086 (BioLegend Cat. No. 500107) AB_315085 (BioLegend Cat. No. 500106)\u003cbr\u003e\nStructure: Cytokine; 17.5 kD (Mammalian)\u003cbr\u003e\nBioactivity: Stimulates T cells, B cells, proliferation\/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes, dendritic cells\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York. 3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello, C. 1996. Blood 87:2095.\u003cbr\u003e\nRegulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin\u003cbr\u003e\nGene ID: 3552\u003cbr\u003e\nUniProt: View information about IL-1alpha on UniProt.org\u003cbr\u003e\nClone: 364-3B3-14\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF\/MNCF)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862380400809,"sku":"500106","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-500106","provider":"Iright","version":"1.0","type":"link"}