{"product_id":"biolegend-503002","title":"Biolegend, 503002, Purified anti-human LT-α (TNF-β) Antibody, 500μg","description":"\u003cp\u003eLymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: E.coli expressed, recombinant human LT-α.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture1,2: The purified 359-238-8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 359-81-11 antibody (Cat. No. 503104) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1,2: The 359-238-8 antibody can neutralize the bioactivity of human LT-α. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503004).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Meager A, et al. 1987. J. Immunol. Methods 104:31. Meager A, et al. 1987. Hybridoma. 6:305.\u003cbr\u003e\nProduct Citations: Ong L, et al. 2016. J Cereb Blood Flow Metab. 10.1177\/0271678X16654920. PubMed\u003cbr\u003e\nRRID: AB_315268 (BioLegend Cat. No. 503002)\u003cbr\u003e\nStructure: TNF superfamily; trimer; 25 kD (Mammalian)\u003cbr\u003e\nBioactivity: Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reac\u003cbr\u003e\nCell Sources: Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells\u003cbr\u003e\nCell Targets: Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells\u003cbr\u003e\nReceptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Aggarwal B, et al. Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc. 3. Bonavida B, et al. Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel. 4. Paul N, et al. 1987. Annu. Rev. Immunol. 6:407.\u003cbr\u003e\nRegulation: Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric\u003cbr\u003e\nGene ID: 4049\u003cbr\u003e\nUniProt: View information about LT-alpha on UniProt.org\u003cbr\u003e\nClone: 359-238-8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862379483305,"sku":"503002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-503002","provider":"Iright","version":"1.0","type":"link"}