{"product_id":"biolegend-503508","title":"Biolegend, 503508, GoInVivo™ Purified anti-mouse\/rat IL-1β Antibody, 25mg","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.\u003cbr\u003e\n25mg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: E. coli -expressed, recombinant mouse IL-1β\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 1.0 EU\/mg of the protein (\u0026lt; 0.1 pg\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The GoInVivo™ antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg\/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg\/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.\u003cbr\u003e\nAdditional Product Notes: GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hogquist K, et al. 1991. J. Immunol. 146:1534. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut) Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture) Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion) Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC) Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB) Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed\u003cbr\u003e\nRRID: AB_2888752 (BioLegend Cat. No. 503507) AB_2888752 (BioLegend Cat. No. 503508) AB_2888752 (BioLegend Cat. No. 503509) AB_2888752 (BioLegend Cat. No. 503510) AB_2888752 (BioLegend Cat. No. 503511) AB_2888752 (BioLegend Cat. No. 503512)\u003cbr\u003e\nStructure: Cytokine; 17.5 kD (Mammalian)\u003cbr\u003e\nBioactivity: Stimulates T cells, B cells, proliferation\/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.\u003cbr\u003e\nRegulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin\u003cbr\u003e\nGene ID: 16176\u003cbr\u003e\nUniProt: View information about IL-1beta on UniProt.org\u003cbr\u003e\nClone: B122\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862548107433,"sku":"503508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-503508","provider":"Iright","version":"1.0","type":"link"}