{"product_id":"biolegend-505301","title":"Biolegend, 505301, Biotin anti-mouse IL-12\/IL-23 p40 (monomer, dimer, heterodimer) Antibody, 50μg","description":"\u003cp\u003eThe C17.8 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19\/p40, as well as p40 monomer and homodimer. The C17.8 antibody can neutralize the bioactivity of natural or recombinant IL-12.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO expressed, recombinant mouse IL-12 p70\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection, ICFC\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg\/ml is recommended. For use as an ELISPOT detection antibody, a concentration range of 0.5-2.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 recombinant protein ranging from 250 to 2 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202\/505208) as capture antibody and recombinant mouse IL-12\/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard. Neutralization1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg). Additional reported applications (for relevant formats of this clone) include: Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis. Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260. Reichmann G, et al. 1999. J. Immunol. 163:3354. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672. Neucath M, et al. 1995. J. Exp. Med. 182:1281. Cousens LP, et al. 1999 J. Exp. Med. 189:1315. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16. O'Connell PJ, et al. 2006. Blood 107:1010. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed\u003cbr\u003e\nProduct Citations: Yan H, et al. 2022. Biomater Adv. 139:213009. PubMed Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed Liang D, et al. 2018. Front Immunol. 8:1801. PubMed Bourquin C, et al. 2009. J Immunol. 183:6078. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed Yan H, et al. 2019. Sci Rep. 9:10447. PubMed Luo X, et al. 2021. Neuron. 109:2691. PubMed\u003cbr\u003e\nRRID: AB_315373 (BioLegend Cat. No. 505301) AB_315374 (BioLegend Cat. No. 505302)\u003cbr\u003e\nStructure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)\u003cbr\u003e\nBioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation\/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me\u003cbr\u003e\nCell Sources: Dendritic cells, monocytes\/macrophages, B cells, T cells\u003cbr\u003e\nCell Targets: T cells, NK cells\u003cbr\u003e\nReceptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.\u003cbr\u003e\nRegulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23\u003cbr\u003e\nGene ID: 16160\u003cbr\u003e\nUniProt: View information about IL-12\/IL-23 p40 on UniProt.org\u003cbr\u003e\nClone: C17.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862940242089,"sku":"505301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-505301","provider":"Iright","version":"1.0","type":"link"}