{"product_id":"biolegend-507215","title":"Biolegend, 507215, PerCP\/Cyanine5.5 anti-human Granzyme A Antibody, 25tests","description":"\u003cp\u003eGranzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified human Granzyme A\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591.\u003cbr\u003e\nProduct Citations: Simmons R, et al. 2013. J Virol. 87:3087. PubMed Meijgaarden K, et al. 2015. PLoS Pathog. 11:1004671. PubMed Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed\u003cbr\u003e\nRRID: AB_2114394 (BioLegend Cat. No. 507215) AB_2114393 (BioLegend Cat. No. 507216)\u003cbr\u003e\nStructure: Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)\u003cbr\u003e\nBioactivity: Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking\u003cbr\u003e\nCell Sources: Cytotoxic T cells, NK cells\u003cbr\u003e\nCell Targets: Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1\/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1\u003cbr\u003e\nReceptors: Assisted by perforin\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Proteases\u003cbr\u003e\nAntigen References: 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679.\u003cbr\u003e\nGene ID: 3001\u003cbr\u003e\nUniProt: View information about Granzyme A on UniProt.org\u003cbr\u003e\nClone: CB9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862968029353,"sku":"507215","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-507215","provider":"Iright","version":"1.0","type":"link"}