{"product_id":"biolegend-508202","title":"Biolegend, 508202, Purified anti-human IL-1β Antibody, 500μg","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-1 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-1 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-1 antibody can neutralize the bioactivity of natural or recombinant IL-1β.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IL-1 beta\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 250 to 2 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA and ELISPOT Capture: The purified JK1B-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JK1B-2 antibody (Cat. No. 508301) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled JK1B-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1β-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Lu Y, et al. 2015. PNAS. 112:607. PubMed\u003cbr\u003e\nProduct Citations: Haug V, et al. 2020. Transplantation. e260:104. PubMed Gruber CN, et al. 2020. Cell. 183:982. PubMed Lu Y, et al. 2015. Proc Natl Acad Sci U S A. 112:607. PubMed Han G, et al. 2022. Ann Transl Med. 10:1134. PubMed\u003cbr\u003e\nRRID: AB_315508 (BioLegend Cat. No. 508201) AB_315509 (BioLegend Cat. No. 508202)\u003cbr\u003e\nStructure: Cytokine; 17.3 kD (Mammalian)\u003cbr\u003e\nBioactivity: Stimulates T cells, B cells, proliferation\/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.\u003cbr\u003e\nRegulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin\u003cbr\u003e\nGene ID: 3553\u003cbr\u003e\nUniProt: View information about IL-1beta on UniProt.org\u003cbr\u003e\nClone: JK1B-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Does this clone recognize the pro or mature form of IL-1 beta?\u003cbr\u003e\nA: The monoclonal anti-human IL-1b antibody clone H1b-98 was generated and screened using the mature form of IL-1b as the immunogen. Since the pro-form contains the mature sequence, it is believed that the antibody also recognize pro-IL-1beta. Similarly, we believe clone JK1B-1 should also recognize both pro and mature forms of IL-1b. However, this information has not been confirmed with in-house testing data.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866458542249,"sku":"508202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-508202","provider":"Iright","version":"1.0","type":"link"}