{"product_id":"biolegend-508801","title":"Biolegend, 508801, Biotin anti-human IL-12\/IL-23 p40 (monomer, dimer, heterodimer) Antibody, 50μg","description":"\u003cp\u003eThe C8.6 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19\/p40, as well as p40 monomer and homodimer. The C8.6 antibody has been reported to strongly inhibit different biological activities of IL-12, (e.g., IFN-gamma induction, mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity). The C8.6 antibody can neutralize the bioactivity of natural or recombinant IL-12.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: COS-expressed p40 subunit of human IL-12\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg\/ml are recommended for each ELISA plate. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg\/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Neutralization1,2,3: The LEAF™Purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-12 bioactivity (Cat. No. 508804).\u003cbr\u003e\nAdditional Product Notes: ELISA or ELISPOT Detection1: The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p40 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the C8.3 antibody (Cat. No. 501702\/501704) as the capture antibody. The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified 7B12 antibody (Cat. No. 511002\/511004) as the capture antibody. Each lot of this antibody is quality control tested by ELISA assay.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. Goodier M, et al. 2000. J. Immunol. 165:139. Aragane Y, et al. 1994. J. Immunol. 153:5366.\u003cbr\u003e\nProduct Citations: Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed Haug V, et al. 2020. Transplantation. e260:104. PubMed Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed\u003cbr\u003e\nRRID: AB_315528 (BioLegend Cat. No. 508801) AB_315529 (BioLegend Cat. No. 508802)\u003cbr\u003e\nStructure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)\u003cbr\u003e\nBioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation\/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me\u003cbr\u003e\nCell Sources: Dendritic cells, monocytes\/macrophages, B cells, T cells\u003cbr\u003e\nCell Targets: T cells, NK cells, PBL\u003cbr\u003e\nReceptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.\u003cbr\u003e\nRegulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23\u003cbr\u003e\nGene ID: 3593\u003cbr\u003e\nUniProt: View information about IL-12\/IL-23 p40 on UniProt.org\u003cbr\u003e\nClone: C8.6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862951973033,"sku":"508801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-508801","provider":"Iright","version":"1.0","type":"link"}