{"product_id":"biolegend-509902","title":"Biolegend, 509902, Purified anti-human IL-1 RA Antibody, 500μg","description":"\u003cp\u003eInterleukin-1 receptor antagonist (IL-1RA) is composed of three isoforms, one secreted (sIL-1Rα\/IL-1Rα1), two intracellular (IL-1Rα2\/IL-1Rα3) and produced by monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratinocytes, and chondrocytes. It is upregulated by IL-1β, IL-4, IL-6 and downregulated by glucocorticoids. The JK1RA-1 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA).\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IL-1 receptor antagonist\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide, 0.02% Proclin 950.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-6 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Capture: The purified JK1RA-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5095 (Cat. No. 509501) antibody as the detecting antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Xi X, et al. 2011. Invest Ophthalmol Vis Sci. 52:4859. PubMed.\u003cbr\u003e\nProduct Citations: Xi X, et al. 2011. Invest Ophthalmol Vis Sci. 52:4859. PubMed\u003cbr\u003e\nRRID: AB_315547 (BioLegend Cat. No. 509901) AB_315548 (BioLegend Cat. No. 509902)\u003cbr\u003e\nStructure: Cytokine; three isoforms, one secreted (sIL-1Rα\/IL-1Rα1), two intracellular (IL-1Rα2\/IL-1Rα3); 17 kD (Mammalian)\u003cbr\u003e\nBioactivity: Storage forms that are released upon cell death to limit inflammation caused by cell debris; competitive inhibitor of IL-1 receptor binding; antagonizes IL-1 activities without having agonist effects; does not initiate intracellular signal transduction pr\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma cells, keratino\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Carter D, et al. 1990. Nature 344:633. 2. Chirivi R, et al. 1993. Cancer Res. 53:5051. 3. Holtkamp G, et al. 1999. Eur. J. Immunol. 29:215. 4. Kim J, et al. 2003. Gene Ther. 10:1543.\u003cbr\u003e\nRegulation: Upregulated by IL-1β, IL-4, IL-6; downregulated by glucocorticoids\u003cbr\u003e\nGene ID: 3557\u003cbr\u003e\nUniProt: View information about IL-1RA on UniProt.org\u003cbr\u003e\nClone: JK1RA-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1 receptor antagonist, IL-1 inhibitor, IL-1 receptor antagonist protein (IRAP), IL-1 inhibitory activity, ILS, MG50, IL-1RN\u003cbr\u003e\nIsotype: Mouse IgG1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866458804393,"sku":"509902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-509902","provider":"Iright","version":"1.0","type":"link"}