{"product_id":"biolegend-512902","title":"Biolegend, 512902, Purified anti-mouse VEGF-A Antibody, 200μg","description":"\u003cp\u003eVEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted glycoproteins that belongs to structurally related superfamily of dimeric growth factors with a cysteine knot motif. This superfamily includes platelet-derived growth factor (PDGF) and transforming growth factor (TGF) family. VEGF-A plays important roles in both vasculogenesis and angiogenesis, including tumor associated angiogenesis and vascular leakage. VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: N-terminal 24 aa sequence of murine VEGF\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Immunohistochemistry: Clone 1F07-2C01 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg\/ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655. Kirsch AH, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed.\u003cbr\u003e\nProduct Citations: Kutikhin AG, et al. 2020. Cells. :9. PubMed Kirsch A, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed Wang L, et al. 2020. Biomed Res Int. 2020:9485398. PubMed\u003cbr\u003e\nRRID: AB_2212504 (BioLegend Cat. No. 512901) AB_2212503 (BioLegend Cat. No. 512902)\u003cbr\u003e\nStructure: cystine-knot growth factors\u003cbr\u003e\nDistribution: aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells\u003cbr\u003e\nFunction: angiogenesis\u003cbr\u003e\nCell Targets: endothelial cells\u003cbr\u003e\nReceptors: VEGFR-1 (Flt-1) and VEGFR-2 (KDR\/Flk-1)\u003cbr\u003e\nCell Type: Macrophages\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: 1. Contreras A, et al. 2007. Am. J. Transpl. 7:155. 2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109. 3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267. 4. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655 5. Ferrara N,et al. 2003. Nat. Med. 9:669. 6. Shibuya M. 2001. Cell Struct. Funct. 26:25. 7. Senger DR, et al. 1983. Science 219:983.\u003cbr\u003e\nRegulation: HIF-1, hypoxiaresponsiveness\u003cbr\u003e\nGene ID: 22339\u003cbr\u003e\nUniProt: View information about VEGF-A on UniProt.org\u003cbr\u003e\nClone: 1F07-2C01\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vascular endothelial growth factor (VEGF), VEGF-A, VEGFA\u003cbr\u003e\nIsotype: Rat IgG, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860857671849,"sku":"512902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-512902","provider":"Iright","version":"1.0","type":"link"}