{"product_id":"biolegend-517104","title":"Biolegend, 517104, Alexa Fluor® 647 anti-mouse IL-17F Antibody, 100μg","description":"\u003cp\u003eInterleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Overexpression of the IL-17F gene in the airways of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo .  P38 MAPK, ERK1\/2, Act1 (NF-kB activator protein 1), and TRAF6 are involved in IL-17F signaling.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Mouse IL-17F-OVA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated 8F5.1A9 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10.1 (Cat. No. 506901 or 506906) antibody as the capture antibody and recombinant mouse IL-17A\/F (Cat. No. 580809) as the standard. Flow Cytometry: The fluorochrome-labeled 8F5.1A9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17F-producing cells within mixed cell populations. Note: For testing mouse IL-17F in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 436107 \u0026amp; 436108) are specially developed and recommended.\u003cbr\u003e\nRRID: AB_2125412 (BioLegend Cat. No. 517104)\u003cbr\u003e\nStructure: Forms a 37 kD homodimer or heterodimer with IL-17A, a IL-17 family member.\u003cbr\u003e\nDistribution: Th17 cells, mast cells, basophils, and epithelial cells.\u003cbr\u003e\nFunction: An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.\u003cbr\u003e\nLigand\/Receptor: IL-17R (CD217)\u003cbr\u003e\nCell Type: Th17, Mast cells, Basophils, Epithelial cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Dong C. 2008. Immunol. Rev. 226:80. 2. Kolls JK, et al. 2004. Immunity 21:467. 3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1. 4. Yang XO, et al. 2008. J. Exp. Med. 205:1063. 5. Ishigame H, et al. 2009. Immunity 30:108. 6. Hu Y, et al. 2010. J. Immunol. 184:4307.\u003cbr\u003e\nGene ID: 257630\u003cbr\u003e\nUniProt: View information about IL-17F on UniProt.org\u003cbr\u003e\nClone: 8F5.1A9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-17F, ML-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862495908009,"sku":"517104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-517104","provider":"Iright","version":"1.0","type":"link"}