{"product_id":"biolegend-518902","title":"Biolegend, 518902, Biotin anti-human IL-17A Antibody, 50μg","description":"\u003cp\u003eIL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Goat\u003cbr\u003e\nImmunogen: Recombinant human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-17A recombinant protein ranging from 1000 to 15.6 pg\/ml are recommended.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated Poly5189 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified BL23 (Cat. No. 512702) antibody as the capture antibody and recombinant human IL-17A (Cat. No. 570509) as the standard.\u003cbr\u003e\nProduct Citations: Nicot S, et al. 2023. Sci Adv. eadi7838:9. PubMed\u003cbr\u003e\nRRID: AB_2561347 (BioLegend Cat. No. 518902)\u003cbr\u003e\nStructure: Cytokine; dimer; ~15 kD (Mammalian)\u003cbr\u003e\nBioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells\u003cbr\u003e\nCell Sources: Activated T cell subpopulation\u003cbr\u003e\nCell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells\u003cbr\u003e\nCross: In sandwich ELISA, cross-reactivity with human IL-17F is negligible and no cross-reactivity with mouse IL-17A is present.\u003cbr\u003e\nReceptors: IL-17R\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41.2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.3. Witowski J, et al. 2007. Kidney Int. 71:514.4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.5. Hymowitz S, et al. 2001. EMBO J. 20:5332.\u003cbr\u003e\nGene ID: 3605\u003cbr\u003e\nUniProt: View information about IL-17A on UniProt.org\u003cbr\u003e\nClone: Poly5189\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)\u003cbr\u003e\nIsotype: Goat Polyclonal Ig\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866164547753,"sku":"518902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-518902","provider":"Iright","version":"1.0","type":"link"}