{"product_id":"biolegend-519102","title":"Biolegend, 519102, Purified anti-human IL-16 Antibody, 100μg","description":"\u003cp\u003eInterleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4 + T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested ELISA, IHC-F, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC) Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA) Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC) Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC) Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)\u003cbr\u003e\nProduct Citations: Colley T, et al. 2016. J Allergy Clin Immunol. 1595:137. PubMed Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed\u003cbr\u003e\nRRID: AB_11204433 (BioLegend Cat. No. 519102)\u003cbr\u003e\nStructure: 12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.\u003cbr\u003e\nDistribution: Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.\u003cbr\u003e\nFunction: Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.\u003cbr\u003e\nLigand\/Receptor: CD4.\u003cbr\u003e\nCell Type: Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Bellon M, et al. 2010. Blood 115:2441. 2. Rofani C, et al. 2009. Stem Cells Dev. 18:671. 3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467. 4. Gu Y, et al. 2008. J. Immunol. 181:4418. 5. McFadden C, et al. 2007. J. Immunol. 179:6439. 6. Qi JC, et al. 2006. Blood 107:135.\u003cbr\u003e\nGene ID: 3603\u003cbr\u003e\nUniProt: View information about IL-16 on UniProt.org\u003cbr\u003e\nClone: 14.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-16\u003cbr\u003e\nIsotype: Mouse IgG2a\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860802162857,"sku":"519102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-519102","provider":"Iright","version":"1.0","type":"link"}