{"product_id":"biolegend-598712","title":"Biolegend, 598712, AZDye 647 Recombinant Human CCL21, 10μg","description":"\u003cp\u003eCCL21 is a CC chemokine highly expressed in lymph nodes. Although it shares only 32% identity with its paralog CCL19, these chemokines bind to the same receptor CCR7. CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 has chemotactic effect for thymocytes and activated T cells, but not for B cells, macrophages and neutrophils. CCL21 is expressed in synovial tissue in rheumatoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and\/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL21 inhibit T cell proliferation and IL-2 secretion. CCL21 has been implicated with periapical periodontitis and scrapie\u003cbr\u003e\n10μg\u003cbr\u003e\nSource: Human CCL21, amino acids Ser24-Pro134 (Accession # O00585) with a C-terminal AZDye647, was expressed in E. coli.\u003cbr\u003e\nMolecular Mass: The 111 amino acid recombinant protein (Ser24-Pro134) has a predicted molecular weight of 14.2 kDa.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by mass spectrometry\u003cbr\u003e\nFormulation: Lyophilized from 0.2 µm filtered solution of protein in DI-water.\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method.\u003cbr\u003e\nConcentration: Lyophilized, 10 µg per vial\u003cbr\u003e\nStorage \u0026amp; Handling: Lyophilized at -20°C to -80°C until expirationLyophilized at 4°C for 6 monthsLyophilized at Room Temperature 1 monthReconstituted at -20°C to -80°C for 12 monthsReconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg\/mL up to 1 mg\/mL in 0.22 µm filtered DI-water. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg\/mL.\u003cbr\u003e\nApplication: FC, Live cell imaging\u003cbr\u003e\nApplication Notes: Protein has been verified for bioactivity prior to conjugation\u003cbr\u003e\nStructure: CCL21 is a 111 amino acids molecule with a predicted molecular weight of 14.2 kDa. PDB ID – 2L4N.\u003cbr\u003e\nDistribution: CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules\u003cbr\u003e\nFunction: CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.\u003cbr\u003e\nInteraction: Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)\u003cbr\u003e\nLigand\/Receptor: CCL21 binds to CCR7 receptor.\u003cbr\u003e\nBioactivity: Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg\/mL.\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Leukocytes, Lymphocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adaptor Proteins, Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nAntigen References: Hedrick JA \u0026amp; Zlotnik A. 1997. J Immunol. 159:1589-93. Reif K, et al. 2002. Nature. 416:94-9. Ziegler E, et al. 2007. J Immunol. 179:6485-93. Pickens SR, et al. 2012. Arthritis Rheum. 64:2471-81.\u003cbr\u003e\nGene ID: O00585\u003cbr\u003e\nUniProt: View information about CCL21 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 6CKine, Secondary lymphoid tissue chemokine (SLC), Exudos 2, Small inducible cytokine subfamily A, member 21 (SCYA21), SLC, TCA4, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 21, Chemokine (C-C Motif) Ligand 21, Chemokine\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866498486441,"sku":"598712","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-598712","provider":"Iright","version":"1.0","type":"link"}