{"product_id":"biolegend-600852","title":"Biolegend, 600852, Purified anti-FosB Antibody, 100μg","description":"\u003cp\u003eFosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full-length recombinant FosB protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg\/mL is suggested. For immunocytochemistry, a concentration range of 5 - 10 μg\/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For ICC, we recommend fixation in 4% PFA and permeabilization for 6 minutes in 0.5% Triton-X. Methanol only and PFA + Methanol fix\/perm methods were tested and produced inferior signal.\u003cbr\u003e\nRRID: AB_2922629 (BioLegend Cat. No. 600851) AB_2922629 (BioLegend Cat. No. 600852)\u003cbr\u003e\nStructure: FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.\u003cbr\u003e\nDistribution: FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.\u003cbr\u003e\nFunction: FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.\u003cbr\u003e\nInteraction: Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G\/C TCA) SWI\/SNF chromatin remodeling complex, DPP4\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Tumor Suppressors\u003cbr\u003e\nAntigen References: Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.\u003cbr\u003e\nGene ID: 2354\u003cbr\u003e\nUniProt: View information about FosB on UniProt.org\u003cbr\u003e\nClone: W19011B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G0\/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866341888169,"sku":"600852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-600852","provider":"Iright","version":"1.0","type":"link"}