{"product_id":"biolegend-601704","title":"Biolegend, 601704, PE anti-BTK Phospho (Tyr223) Antibody, 100tests","description":"\u003cp\u003eBruton’s tyrosine kinase (BTK) is a Tec family kinase that plays a critical role in B cell development. Upon B cell receptor engagement, BTK translocates to the plasma membrane, where it is transphosphorylated by LYN and SYK kinases at Tyr 551. This initial phosphorylation event is followed by autophosphorylation at Tyr 223. Phosphorylated Tyr 223 is proposed to serve as a docking site for other proteins containing an SH2 domain. Downregulation of BTK activity is achieved through phosphorylation at Ser 180 by PKCβ, which results in reduced membrane recruitment and concomitant transphosphorylation. BTK-mediated B cell receptor signaling is required for B cell survival in the bone marrow and is a therapeutic target for certain B cell malignancies. Mutations in the BTK gene results in X-linked agammaglobulinemia (XLA), a primary immunodeficiency disease characterized by a failure in B cell maturation. Additionally, BTK plays a role in Toll-like receptor signaling in innate immune cells, and has been shown to play a direct role in NLRP3 inflammasome activation, thus contributing to ischaemic brain injury.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human BTK peptide phosphorylated at Tyr 223, Complete Freund's adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Intracellular Flow Cytometry Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: A16128B was generated in the same immunization that produced clone A16128C (anti-BTK Phospho (Tyr223)\/ITK Phospho (Tyr180)). Both clones bind BTK Phospho Tyr223 with similar affinity, but in comparison to clone A16128C, clone A16128B has weaker recognition of phosphorylated Tyrosine 180 of ITK by intracellular staining and flow cytometric analysis.\u003cbr\u003e\nProduct Citations: Purvis GSD, et al. 2022. Br J Pharmacol. 179:2754. PubMed\u003cbr\u003e\nRRID: AB_2721620 (BioLegend Cat. No. 601703) AB_2721621 (BioLegend Cat. No. 601704)\u003cbr\u003e\nStructure: BTK encodes a 659 amino acid protein with a predicted molecular weight of 76.2 kD. Contains a PH domain (a.a. 1-133) that binds phosphatidylinositol (3,4,5)-triphosphate (PIP3) and polyphosphates, an SH3 domain (a.a. 214-274), an SH2 domain (a.a. 281-377), and a protein kinase domain (402-655).\u003cbr\u003e\nDistribution: Cytosol, plasma-membrane associated\u003cbr\u003e\nFunction: Links B cell receptor engagement with downstream signaling events\u003cbr\u003e\nInteraction: ARID3A, BLNK, CAV1, GNAQ, GTF2I, PLGC2, PRKD1, SH3BP5\u003cbr\u003e\nLigand\/Receptor: PIP3\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Krysiak K, et al. 2017. Blood. 129:473. Burger JA, et al. 2016. Nat Commun. 7:11589. Wang Q, et al. 2015. Elife. 4:e06074. Ito M, et al. 2015. Nat. Commun. 6:7360. Pillinger G, et al. 2015. Sci. Rep. 5:12949. Cinar M, et al. 2013. Leuk. Res. 37:1271. Doyle SL, et al. 2007 J. Biol. Chem. 282:36953. Nore BF, et al. 2003. Biochem. Biophys. Acta. 1645:123. Li Z, et al. 1997. Proc. Natl. Acad. Sci. 94:13820.\u003cbr\u003e\nGene ID: 695\u003cbr\u003e\nUniProt: View information about BTK Phospho Tyr223 on UniProt.org\u003cbr\u003e\nClone: A16128B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bruton’s tyrosine kinase, Bruton agammaglobulinemia tyrosine kinase, AGMX1, ATK, IMD1, PSCTK1, XLA, BPK\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864401924265,"sku":"601704","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-601704","provider":"Iright","version":"1.0","type":"link"}