{"product_id":"biolegend-602252","title":"Biolegend, 602252, Purified anti-EGFR Phospho (Tyr845) Antibody, 200microl","description":"\u003cp\u003eEpidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 845, which is found in the kinase activation loop, stabilizes the active conformation and leads to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.\u003cbr\u003e\n200microl\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 845\u003cbr\u003e\nFormulation: PBS\/0.09% NaN3\/50% glycerol\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted at -20°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:2000. For immunocytochemistry, a dilution of 1:40 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: During product development testing for western blot, clone Poly21A03 displayed high sensitivity. We recommend using Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) for detection, and using shorter primary incubation times. For ICC, we recommend using 4% PFA-fixed cells permeabilized with Triton X-100 or methanol.\u003cbr\u003e\nRRID: AB_2910475 (BioLegend Cat. No. 602251) AB_2910475 (BioLegend Cat. No. 602252)\u003cbr\u003e\nStructure: EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.\u003cbr\u003e\nDistribution: Plasma membrane\/Ubiquitously expressed\u003cbr\u003e\nFunction: EGFR signaling, cell signaling\u003cbr\u003e\nLigand\/Receptor: EGF\u003cbr\u003e\nAntigen References: Hunter T and Cooper JA. 1981. Cell. 24:741-52. Gill GN and Lazar CS. 1981. Nature. 293:305-7. Reynolds FH, et al. 1981. Nature. 292:259-62. Biscardi JS, et al. 1999. J Biol Chem. 274:8335-42. Zhou M, et al. 2013. Cancer Res. 23:7056-67-67.\u003cbr\u003e\nGene ID: 1956\u003cbr\u003e\nUniProt: View information about EGFR Phospho Tyr845 on UniProt.org\u003cbr\u003e\nClone: Poly21A03\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866161533097,"sku":"602252","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-602252","provider":"Iright","version":"1.0","type":"link"}