{"product_id":"biolegend-603702","title":"Biolegend, 603702, Purified anti-STAT1 Antibody, 100μg","description":"\u003cp\u003eSTAT1, also known as signal transducter and activator of transcription 1, is a ubiquitously expressed protein that is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, cytosolic STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcriptional activator. Two isoforms of STAT1, with predicted molecular weights of 87 (STAT1α) and 83 kD (STAT1β), exist as a result of alternative RNA processing. This clone is specific for STAT1α. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human STAT1 peptide phosphorylated at Serine 727.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 µg per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody is specific for STAT1 isoform a and is not predicted to recognize the ß isoform due to the absence of the region corresponding to the immunizing peptide.This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).U3A cells were selected for antibody specificity testing because the cell line does not express STAT11.This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Muller M, et al. 1993. EMBO J. 12:4221\u003cbr\u003e\nProduct Citations: Kiritsy MC, et al. 2021. Elife. 10:. PubMed\u003cbr\u003e\nRRID: AB_2749867 (BioLegend Cat. No. 603701) AB_2749868 (BioLegend Cat. No. 603702)\u003cbr\u003e\nStructure: STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.\u003cbr\u003e\nDistribution: Ubiquitously expressed; cytosolic and nuclear distribution\u003cbr\u003e\nFunction: Transcription activator\/Cytokine Signaling\u003cbr\u003e\nInteraction: Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: Durbin JE, et al. 1996. Cell 8:443. Darnell JE Jr, et al. 1994. Science 264:1415. Chen X, et al. 1998. Cell 93:827. Ramana CV, et al. 2000. Oncogene 19:2619.\u003cbr\u003e\nGene ID: 6772\u003cbr\u003e\nUniProt: View information about STAT1 on UniProt.org\u003cbr\u003e\nClone: A15158C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 1, Transcription factor ISGF-3 components p91\/p84\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864391078057,"sku":"603702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-603702","provider":"Iright","version":"1.0","type":"link"}