{"product_id":"biolegend-604951","title":"Biolegend, 604951, Purified anti-ERK1\/2 Phospho (Thr202\/Tyr204) Antibody, 50microl","description":"\u003cp\u003eERK1\/2 (extracellular signal-regulated protein kinase) are members of the mitogen-activated protein kinase (MAPK) family of serine\/threonine kinases. ERK1, also called Mitogen-activated protein kinase 3 (MAPK3) and ERK2, also called Mitogen-activated protein kinase 1 (MAPK1), are both integral effectors of the Ras-dependent extracellular signal-regulated kinase 1 (ERK1\/2) mitogen-activated (MAP) kinase pathway. As such, they are vital regulators of cell cycle, differentiation, proliferation, stress-response, survival, and senescence. ERK1\/2 are activated via phosphorylation of their tyrosine (Tyr204) and threonine (Thr202) residues by MEK1 and MEK2 in response to mitogenic stimuli. Upon activation, ERK1\/2 translocate to the nucleus where they function in the regulation of transcription and translation. Dysregulation of ERK1\/2 and the hyper-activation of the ERK pathway are characteristics of many cancers where it contributes to tumorigenesis, metastasis, and treatment-resistance, making ERK1\/2 an important therapeutic target.\u003cbr\u003e\n50microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Synthetic peptide of human Erk1\/2 phosphorylated at Thr202\/Tyr204\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Use 20 μL per 10 mL antibody dilution buffer for each gel.\u003cbr\u003e\nApplication Notes: This clone was tested by ICC in HeLa cells treated with or without 100 ng\/mL PMA. Three fix\/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. All three methods resulted in weak staining and a lack of phospho-specificity.\u003cbr\u003e\nRRID: AB_2927813 (BioLegend Cat. No. 604951) AB_2927813 (BioLegend Cat. No. 604952)\u003cbr\u003e\nStructure: ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD. They share 85% homology.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/ Localized to the cell membrane and nucleus\u003cbr\u003e\nFunction: Serine\/threonine-protein kinase, phosphatase\u003cbr\u003e\nInteraction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1\/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Busca R, et al. 2016. Front. Cell Dev. Biol. 4:53. Meloche S and Pouyssegur J. 2007. Oncogene. 26:3227. Wortzel I and Seger R. 2011. Genes and Cancer. 3:195.\u003cbr\u003e\nGene ID: 55945595\u003cbr\u003e\nUniProt: View information about ERK1\/2 on UniProt.org\u003cbr\u003e\nClone: Poly21A07\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Extracellular signal-regulated kinase 1\/2, Mitogen-activated protein kinase MAPK3\/MAPK1, p44ERK1\/p42ERK2, p44-MAPK\/p42MAPK, PRKM3\/PRKM2, ERK\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866161696937,"sku":"604951","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-604951","provider":"Iright","version":"1.0","type":"link"}